Zastosowanie cytofluorymetru przepływowego do liczenia komórek drożdży w płynie hodowlanym

• Autorzy: Zbigniew Garncarek , Jaromir Fiala , Chris Kent

Warianty tytułu

A Flow-cytometric Method for Counting of Yeast Cells in Fermentation Broth

• Języki publikacji: PL

Abstrakty

Celem pracy jest określenie przydatności cytofluorymetru przepływowego do liczenia martwych i żywych komórek drożdży, z jednoczesnym eliminowaniem zanieczyszczeń próby. (fragment tekstu)

EN

A simple procedure has been developed for the number and viability of yeast cells in fermentation broth by the use of flow cytometry. Propidium iodide was used for viability determination and fluorescent beads was added to cell suspension as an internal standard. Absolute counts of yeast cells were calculated by determining the ratio of beads to the cell population of interest and then multiplying this ratio by the number of beads in the tube. It was found that the number of beads and the cells should be almost equal in the tube. The new method was found to be comparable to that of hemacytometr with high precision. (original abstract)

Słowa kluczowe

PL

Chemia organiczna; Technologia; Dobór próby badawczej

EN

Organic chemistry; Technology; Selection of test methods

• Czasopismo: Prace Naukowe Akademii Ekonomicznej we Wrocławiu. Technologia
• Rocznik: 2000
• Tom: 6
• Numer: nr 833
• Strony: 19--26

Twórcy

autor

Zbigniew Garncarek

• Akademia Ekonomiczna we Wrocławiu

autor

Jaromir Fiala

• Wyższa Szkoła Chemiczno-Techniczna, Praga

autor

Chris Kent

• The University of Birmingham, School of Chemical Engineering, Birmingham, Wielka Brytania

Bibliografia

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