Application of Toxicological Assay and Spectrophotometric Measurements in Evaluation Safety of Textiles

• Autorzy: Renata Salerno-Kochan

Warianty tytułu

Zastosowanie metod toksykologicznych i pomiarów spektrofotometrycznych w określaniu bezpieczeństwa wyrobów tekstylnych

• Języki publikacji: EN

Abstrakty

EN

A new way of detection chemical substances included in textiles based on toxicological assay and optical density measurement method in a presence of Tetrahymena pyriformis as bioindicator is proposed. The experiment was carried out in order to assess the toxicity level of analyzed substances in relation to the chosen organisms, as well. Formaldehyde, as a tested substance was used. The Tetrahymena pyriformis culture was added to water solutions of formaldehyde within the range 1.5-50 mg/L and incubated at 28oC for 24 hours. The changes in proliferation of Tetrahymena pyriformis in formaldehyde solutions were assessed based on direct readings of optical density, measured at 330 nm after 4. 6. 8 and 24 hours using UV-VIS spectrophotometer. The performed experiments showed that proposed method enables to detect a very small amount of formaldehyde in water solutions and that the toxicity of formaldehyde is stronger than expected. (original abstract)

W artykule zaprezentowano wyniki badań eksperymentalnych nad możliwością zastosowania badań toksykologicznych opartych na Tetrahymenie pyriformis pełniącej rolę bioindykatora oraz pomiarów gęstości optycznej hodowli do oznaczenia obecności substancji szkodliwych zawartych w produktach włókienniczych. Badania miały także na celu określenie poziomu toksyczności analizowanych substancji w odniesieniu do wybranych organizmów testowych. Jako substancję testową zastosowano formaldehyd. Badania polegały na inkubowaniu hodowli komórek Tetrahymena pyriformis w wodnych roztworach formaldehydu o stężeniach 1,5-50 mg/l przez 24 h, w temperaturze 280C. Rozwój komórek testowych w roztworach formaldehydu analizowano w oparciu o pomiary gęstości optycznej będącej miarą proliferacji komórek, po 4, 6, 8 i 24 h z zastosowaniem spektrofotometru UV-VIS, przy długości fali 330 nm. Badania wykazały możliwość wykrycia bardzo niewielkich ilości formaldehydu w roztworach wodnych oraz że szkodliwość formaldehydu w roztworach jest większa, niż jest to powszechnie uznawane. (abstrakt oryginalny)

Słowa kluczowe

EN

Commodity science; Industrial commodities; Textile products; Safety of products; Commodity research

PL

Towaroznawstwo; Towaroznawstwo przemysłowe; Wyroby włókiennicze; Bezpieczeństwo produktów; Badania towaroznawcze

• Czasopismo: Towaroznawcze Problemy Jakości
• Rocznik: 2010
• Numer: nr 3
• Strony: 67--76

Twórcy

autor

Renata Salerno-Kochan

• Cracow University of Economics, Poland

Bibliografia

• [1] Agents Classified by the IARC Monographs, The International Agency for Research on Cancer (IARC) http://monographs.iarc.fr/ENG/Classification/index.php
• [2] Beermann K., Buschmann H.-J., Schollmeyer E. (1999) A calorimetric method for the rapid evaluation of toxic substances using Tetrahymena pyriformis, Thermochemica Acta, 337, 65-69.
• [3] Bogaerts P., Bohatier J., Bonnemoy F. (2001) Use of the Ciliated Protozoan Tetrahymena pyriformis for the Assessment of Toxicity and Quantitive Structure-Activity Relationships of Xenobiotics: Comparison with Microtox Test, Ecotoxicology and Environmental Safety, 49, 293-301.
• [4] Chemistry of the Textiles Industry, ed. C.M. Carr, Blackie Academic & Professional, : London - Glasgow - Weinheim - New York - Tokyo - Melbourne - Madras, 1995.
• [5] Commission Decision 2009/567/EC of 9 July 2009 establishing the ecological criteria for the award of the Community Ecolabel for textile products, OJ L 197/70.
• [6] Council Directive of 27 July 1976 on the approximation of the laws, regulations and administrative provisions of the Member States relating to restrictions on the marketing and use of certain dangerous substances and preparations, (76/769/EEC), OJ L 262, 27.9.1976,201.
• [7] Graf W. (1985) GIT Fachzeitschrift fuer das Laboratorium, 29, 601.
• [8] Hellung-Larsen P., I. Lyhne. A.P., Andersen and U. Koppelhus (1993) Characteristics of dividig and non-dividing Tetrahymena cells at different physiological states, Eur. J. Protistol., 29, 182-190.
• [9] Knittel D., Beermann K., Schollmeyer E. (2003) Surface of Textiles and the Human Skin. 2. Testing Methods for Skin Compatibility of Textiles, Textiles Ingredients and Textile Auxiliares, Exogenous Dermatology, 2, 17-22.
• [10] Larsen J., Schultz T.W., Rasmussen L., Hooftman R., Pauli W. (1997) Progress in an Ecotoxicological Standard Protocol with Protozoa: Results From a Pilot Ringtest with Tetrahymena Pyriformis, Chemosphere, 35, 5, 1023-1041.
• [11] Mosmann T. (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Meth., 65, 55-65.
• [12] Nałęcz-Jawecki G. (2003) Water Environment Toxicity Investigated by Bioassays, Biuletyn Wydziału Farmaceutycznego, AMW, 2 (in Polish).
• [13] Ordinance of the Council of Ministers on safety and labelling textile materials, 6th April 2004, Dz. U. No. 81, item 743, with further amendments (in Polish).
• [14] Öko Standard 100. Limit Values and Fastness. Edition 01.01.2010, www.oeko-tex.com
• [15] PN-EN ISO 14184-1:2001 Textiles - Determination of formaldehyde - Part 1: Free and hydrolyzed formaldehyde (water extraction method).
• [16] PN EN 14362-2:2005 Textiles Methods for the determination of certain aromatic amines derived from azo colorants Part 2: Detection of the use of certain azo colorants accessible by extracting the fibres.
• [17] PN-EN ISO 17070: 2007. Leather. Chemical tests. Determination of pentachlorophenol content.
• [18] PN EN ISO 71-3: 1998. Safety of toys: Specification for migration of certain elements.
• [19] Rodriguez-Mozaz S., Lopez de Aida M.J., Barcelo D. (2006) Biosensors as useful tools for environmental analysis and monitoring, Anal. Bioanal. Chem., 386, 1025-1041.
• [20] Rouette M.K. (2001) Encyclopedia of Textile Finishing, Springer-Verlag-Berlin-Heidelberg-New York.
• [21] Sauvant M.P., Pepin D., Bohatier J., Groliere C.A. (1995) Microplate Technique for Screening and Assessing Cytotoxicity of Xenobiotics with Tetrahymena pyriformis, Ecotoxicology and Environmental Safety, 32, 159-165.
• [22] Sauvant M.P., Pepin D. Piccinni E. (1999) Tetrahymena pyriformis: A Tool for Toxicological Studies. A Review, Chemosphere, 38 (7), 1631-1669.
• [23] Zilberg D., Sinai T. (2006) Optimalization and validation of a colorimetric assay for Tetrahymena sp. survival, Res. Microbiol., 157, 355-359.