The effects of inorganic salts on biosynthesis of pectinolytic enzymes by Aspergillus Niger

• Autorzy: Kiro Mojsov
• Języki publikacji: EN

Abstrakty

EN

The paper examines effects of different inorganic salts in an apple pulp base on the production of pectinolytic enzymes with the aim of optimizing the medium for maximal enzyme production. The apple pulp combined with corn flour and simple mineral salts was used as a nourishing base in submerged production of pectinolytic enzymes by the fungus Aspergillus niger MK-15. The growth of the microorganism (dry weight) on different sources of nitrogen showed maximum dry weight with (NH4)2HPO4. The growth of the microorganism (dry weight) on different concentration of (NH4)2HPO4 (by 0.2% to 0.8%) provided maximal dry weight with 0.7% (NH4)2HPO4. The different inorganic salts (sources of nitrogen) on base stimulated the production of pectinolytic enzymes and enhanced by up to twofold the growth of Aspergillus niger in submerged fermentation. The best source of nitrogen on base was (NH4)2HPO4 with optimal concentration of 0.7%. The obtained results represent practical importance for using apple pulp as a carbon source for production of pectinolytic enzymes in submerged fermentation.(original abstract)

Słowa kluczowe

EN

Inorganic chemistry; Scientific experiment; Chemical compounds

PL

Chemia nieorganiczna; Eksperyment badawczy; Związki chemiczne

• Czasopismo: Perspectives of Innovations, Economics and Business
• Rocznik: 2010
• Tom: 4
• Numer: nr 1
• Strony: 109--112

Twórcy

autor

Kiro Mojsov

• Goce Delcev University in Stip, Macedonia

Bibliografia

• Aguilar, G.   and   Huitron, C., 1986.  "Application   of   fed-batch  cultures  in  the   production of       extracellular pectinases by Aspergillus sp.",  Enzyme and Microbial Technology, Vol. 9, pp.541-45
• Alkorta, I., Garbisu, C., Liama, J., Sera, J., 1998. "Industrial applications of pectic enzymes: A review", Process Biochemistry, Vol.33, pp.21-28
• Archer, P., Roberts, L. and MacKenzie, B., 1990. "Heterologous protein secretion from Aspergillus niger in phosphate-buffered batch culture", Appl. Microbiology and Biotechnology, Vol.34, pp. 313-15
• Bailey, M. and Pessa, E., 1990.  "Strain and process for production of polygalacturonase", Enzyme and  Microbial Technology, Vol.12, pp.266-71
• Berovič, M. and Ostroveršnik, H.,  1997. "Production of  Aspergillus  niger   pectolytic  enzymes  by solid state bioprocessing of apple pomace", Journal of Biotechnology, Vol.53, pp.47-53
• Fogarty, W. and Kell, C., 1983. "Pectic enzymes", in: Fogarty,W. (Ed.), "Microbial Enzymes and Biotechnology", Elsevier Applied Science,  London, pp. 131-82
• Friedrich, J.,  Cimerman, A., Steiner, W., 1989.   "Submerged   production of  pectolytic enzymes by Aspergillus niger: Effect of different aeration /agitation regimes", Applied Microbiology and     Biotechnology, Vol. 31, pp. 490-94
• Kleinman, M., Evans, I.H. & Beran, E.A., 1988. "Sodium phosphate enhancement of starch hydrolysis by a diastatic strain of Saccharomyces cereuisiae", Biotechnology Letters, Vol. 10, pp.825-28
• Larios, G., García, J. and Huítrón, C., 1989. "Endo-polygalacturonase production from ntreated lemon peel by Aspergillus sp. CH-Y-1043", Biotechnology Letters, Vol.11, pp.729-34
• Leuchtenberger, A., Friese, E. and Ruttloff, H., 1989. "Variation of polygalacturonase and  pectinesterase synthesis by aggregated mycelium of Aspergillus niger in dependence on the carbon    source", Biotechnology Letters, Vol.11, pp.255-58
• Maldonado, M., Navarro, A., Calleri, D., 1986. "Production of pectinases by Aspergillus sp.  using differently pretreated lemon peel as the carbon source", Biotechnol. Lett, Vol. 8(7), pp.501-504
• Peričin, D., Jarak, M., Antov, M., Vujičič, B., Kevrešan, S., 1992.  "Effect of inorganic phosphate on    the secretion of pectinolytic enzymes by Aspergillus niger", Letters in Applied  Microbiology, Vol.14, pp.275-78
• Pilknik, W. and Voragen, G., 1993. "Pectic enzymes in fruit and vegetable juice manufacture, in:  Nagodawithana, T. and Reed, G. (Eds.), "Enzyme and food processing",  Academic     Press, London, pp.363
• Schmidth, O., Angermann, H., Frommhold-Treu, I., Hoppe, K. 1995. "Experimental and theorical investigation  of  submerged  fermentation and synthesis of pectinolytic enzymes by Aspergillus niger", Appl. Microbiol. Biotechnol.Vol. 43, pp.424-30