Metody skriningu bakteriocynogennych bakterii fermentacji mlekowej

• Autorzy: Anna Sip , Maria Krasowska , Michał Więckowicz , Włodzimierz Grajek

Warianty tytułu

Methods to Screen Bacteriocinogenic Lactic Acid Bacteria

• Języki publikacji: PL

Abstrakty

W pracy omówiono metody poszukiwania bakterii fermentacji mlekowej zdolnych do syntezy bakteriocyn. Przedstawiono trzy tradycyjne procedury skriningowe. Opisano także sposoby identyfikacji LAB oraz detekcji wytwarzanych przez nie bakteriocyn. Zwrócono uwagę na złożony (wielopłaszczyznowy) charakter tradycyjnych procedur skriningowych i wyjaśniono przyczyny niskiej ich efektywności. Omówiono ponadto rolę analizy metagenomowej oraz fluorescencyjnej hybrydyzacji in situ w pracach skriningowych. Podkreślono, że techniki te mogą być stosowane we wstępnych etapach skriningu do typowania środowisk bogatych w bakteriocynogenne LAB, zwiększając tym samym prawdopodobieństwo pozyskania ze środowiska naturalnego drobnoustrojów zdolnych do syntezy aktywnych bakteriocyn. W końcowej części pracy dokonano porównania tradycyjnych i nowoczesnych procedur skriningowych. (abstrakt oryginalny)

EN

In this paper, some screening methods to detect LAB able to synthesize bacteriocins were discussed. Three conventional screening procedures were presented. Furthermore, the methods to identify LAB and to detect bacteriocins produce by LAB were described. The complex (multipronged) character of conventional screening procedures was emphasized and the reasons for their low effectiveness were explained. In addition, the role of both the metagenomic analysis and the fluorescent hybridization in situ in screening procedures was detailed. It was emphasized that those techniques might be applied at initial screening stages to determine habitats rich in bacteriocinogenic LAB, thereby increasing the probability of isolating the bacteriocin-producing microorganisms from natural systems. In the final part of the paper, the conventional and novel screening procedures were compared. (original abstract)

Słowa kluczowe

PL

Żywność; Jakość produktów żywnościowych; Mikrobiologia; Badanie żywności; Biotechnologia

EN

Food; Quality of food products; Microbiology; Food research; Biotechnology

• Czasopismo: Żywność: nauka - technologia - jakość
• Rocznik: 16 (2009)
• Numer: nr 1 (62)
• Strony: 5--26

Twórcy

autor

Anna Sip

• Uniwersytet Przyrodniczy w Poznaniu

autor

Maria Krasowska

• Uniwersytet Przyrodniczy w Poznaniu

autor

Michał Więckowicz

• Uniwersytet Przyrodniczy w Poznaniu

autor

Włodzimierz Grajek

• Uniwersytet Przyrodniczy w Poznaniu

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