Produkcja naringinazy przez Aspergillus niger - optymalizacja składu podłoża

• Autorzy: Joanna Bodakowska-Boczniewicz , Zbigniew Garncarek

Warianty tytułu

Production of Naringinase by Aspergillus Niger - Culture Medium Optialization

• Języki publikacji: PL

Abstrakty

Celem przeprowadzonych badań był wybór szczepu Aspergillus niger o dużej zdolności do syntezy naringinazy oraz optymalizacja składu podłoża hodowlanego. Spośród przebadanych szczepów A. niger największą zdolność do biosyntezy naringinazy wykazywał szczep KMS, który hodowano na podłożu płynnym z dodatkiem naringiny jako czynnika indukującego. Optymalizowano, metodą czynnikowo-gradientową Boxa i Wilsona, stężenie pięciu składników podłoża: azotanu sodu, ekstraktu drożdżowego, diwodorofosforanu potasu, glukozy oraz naringiny. Przeprowadzone badania pozwoliły na ustalanie optymalnych stężeń następujących czynników: 0,78 g × 100 ml-1 NaNO3; 2 g × 100 ml-1 ekstraktu drożdżowego; 0,157 g × 100 ml-1 KH2PO4; 0,22 g × 100 ml-1 glukozy oraz 120 mg × 100 ml-1 naringiny(abstrakt oryginalny)

EN

The purpose of the study was the selection of A. niger strain for the biosynthesis of naringinase and the optimization of culture medium composition. A. niger KMS strain was recognised as best for the enzyme biosynthesis. A. niger KMS was grown on liquid medium with naringin as an inducer. Optimization was carried out by Box-Wilson method. The concentration of sodium nitrate, yeast extract, potassium dihydrogen phosphate, glucose and naringin was optimized. The optimal concentration of the components were: 0.78 g × 100 ml-1 NaNO3; 2 g × 100 ml-1 yeast extract; 0.157 g × 100 ml-1 KH2PO4; 0.22 g × 100 ml-1 glucose and 120 mg × 100 ml-1 naringin(original abstract)

Słowa kluczowe

PL

Biosynteza; Optymalizacja

EN

Biosynthesis; Optimalization

• Czasopismo: Prace Naukowe Uniwersytetu Ekonomicznego we Wrocławiu
• Rocznik: 2016
• Numer: nr 461 Wybrane zagadnienia z bioekonomii
• Strony: 19--29

Twórcy

autor

Joanna Bodakowska-Boczniewicz

• Uniwersytet Ekonomiczny we Wrocławiu

autor

Zbigniew Garncarek

• Uniwersytet Ekonomiczny we Wrocławiu

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Identyfikatory

DOI

10.15611/pn.2016.461.02