Zastosowanie chromatografii jonowej sprzężonej z pulsacyjną detekcją amperometryczną do oznaczania zawartości mio-inozytolu w materiałach paszowych pochodzenia roślinnego

• Autorzy: Robert Duliński , Anna Starzyńska-Janiszewska

Warianty tytułu

Application of Ion Chromatography Coupled With Pulse Amperometric Detection to Determine Myo-inositol Content in Plant Components of Feed

• Języki publikacji: PL

Abstrakty

W pracy zastosowano technikę chromatografii jonowej do oznaczania zawartości mio-inozytolu w roślinnych komponentach pasz, jako alternatywę testu mikrobiologicznego. Materiał badawczy stanowiły: pszenica, kukurydza i soja niemodyfikowane oraz odmiany GMO, a także przygotowane na ich bazie mieszanki paszowe. Oznaczono inozytol: całkowity, wolny oraz uwolniony w procesie in vitro, symulującym przewód pokarmowy drobiu. Mio-inozytol został rozdzielony w wysoko sprawnej kolumnie aniono-wymiennej CarboPack PA100 i oznaczony w trybie pulsacyjnej detekcji amperometrycznej (ang. high performance anion-exchange chromatography with pulse amperometric detection, HPAEC-PAD). Całkowity inozytol oznaczony techniką HPAEC-PAD kształtował się na poziomie od 2572 μg/g (pasza kukurydziano-sojowa) do 3667 μg/g (kukurydza). Otrzymane dane były porównywalne z testem mikrobiologicznym tylko w przypadku śruty sojowej, zarówno w formie niemodyfikowanej, jak i GMO (odpowiednio: 2392 i 2636 μg/g). W odniesieniu do pozostałych komponentów pasz wyniki uzyskane za pomocą techniki HPAEC-PAD były wyższe od rezultatów testu mikrobiologicznego, średnio o 30 - 50 %. Korelacja pomiędzy obiema metodami w zakresie stężeń 0,1-100 μg/ml była najwyższa w przypadku analizy mio inozytolu uwolnionego w procedurze trawienia in vitro (r = 0,88). W pozostałych przypadkach, w teście mikrobiologicznym uzyskiwano systematycznie niższe wyniki, ponieważ uwolnienie całkowitego inozytolu przez kwaśną hydrolizę było niepełne i testowy mikroorganizm S.cerevisiae ATCC 9080 nie mógł wykorzystać do wzrostu monofosforanu inozytolu. Inna możliwość to efekt dużej zawartości aminokwasów hydrofobowych w komponentach pasz zawierających soję, które mogą interferować z systemem pulsacyjnej detekcji amperometrycznej. (abstrakt oryginalny)

EN

In the research project, an ion chromatography technique was used as an alternative to microbiological assay to determine the content of myo-inositol in plant components of feeds. The analyzed material consisted of both genetically non-modified and modified varieties of wheat, corn, and soybean, as well as of feed mixtures made on the basis thereof. The contents of total and free inositol were determined as was the content of dialyzable inositol released by in vitro procedure to simulate the intestinal tract of broilers. The myo-inositol was separated on a high-performance, anion-exchange CarboPack PA100 column, and determined using a pulsed amperometric detection procedure (i.e. high performance anion-exchange chromatography with pulse amperometric detection, HPAEC-PAD). The content of total inositol determined by the HPAEC-PAD method ranged between 2572 μg/g (corn-soybean feed) and 3667 μg/g (corn). The data obtained were comparable only with the results of the microbiological assay of both the genetically non- modified and modified soybean pellets, (2392 and 2636 μg/g, respectively). As regards the other feed components, the results obtained by the HPAEC-PAD method were higher than those obtained using the microbiological method, on average: by 30 to 50 %. In the range of the concentration values from 0.1 to 100 μg/ml, a correlation between the two methods analyzed was the highest in the case of the analysis of myo-inositol released during the in vitro digestion procedure (r = 0.88). In all other cases, the results of the microbiological assay were systematically lower because the liberation of total inositol by the acid hydrolysis was incomplete, and S. cerevisiae ATCC 9080, the micro-organism analyzed, couldn't utilize the inositol monophosphate to grow. The other possibility was a common presence of hydrophobic aminoacids in soy-containing components of feeds, which could interfere with the system of pulsed amperometric detection. (original abstract)

Słowa kluczowe

PL

Biochemia; Chemia spożywcza

EN

Biochemistry; Food chemistry

• Czasopismo: Żywność: nauka - technologia - jakość
• Rocznik: 18 (2011)
• Numer: nr 3 (76)
• Strony: 173--185

Twórcy

autor

Robert Duliński

• Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie

autor

Anna Starzyńska-Janiszewska

• Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie

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