UVA Radiation Enhancement of β-carotene Yields in Rhodotorula mucilaginosa

• Autorzy: Ludmiła Bogacz-Radomska , Joanna Harasym

Warianty tytułu

Zwiększenie produkcji beta-karotenu w drożdżach Rhodotorula mucilaginosa pod wpływem promieniowania UVA

• Języki publikacji: EN

Abstrakty

EN

The influence of UVA and VIS radiation on the β-carotene biosynthesis by yeast Rhodotorula mucilaginosa was studied. The impact of radiation characteristic on biomass growth, substrate consumption and β-carotene production in submerged batch culture in bioreactor was studied. Three different types of light sources were used in the experiment: VIS - 15 W, wavelength range from 400 to 800 nm, the largest emission at 420 nm; UVA - 18 W, blue light, wavelength range of 350 to 430 nm, the largest emission at 370 nm; UVA - 20 W, wavelength range from 350 to 575 nm, the largest emission at 350 nm. In cultures conducted with UVA - 18 W radiation the highest β-carotene and carotenoids cellular concentration were achieved, respectively 63.20 μg/gd.w. and 0.97 mg/gd.w.. In cultures subject to VIS radiation a lower β-carotene concentration at 43.60 μg/gd.w. and the total carotenoids concentration at 0.83 mg/gd.w. was obtained. The ultraviolet radiation with the emission peak of radiation at 370 nm promoted the β-carotene and carotenoids production in yeast Rhodotorula mucilaginosa.(original abstract)

Badano wpływ promieniowania UVA i VIS na biosyntezę β-karotenu przez drożdże Rhodotorula mucilaginosa. Określano wpływ promieniowania charakterystycznego na wzrost biomasy, zużycie substratu i produkcję β-karotenu we wgłębnej hodowli okresowej prowadzonej w bioreaktorze. Zastosowano trzy różne rodzaje źródeł światła: VIS - 15 W, zakres długości fali od 400 do 800 nm, największa emisja przy 420 nm; UVA1 - 18 W, zakres długości fali od 350 do 430 nm, największa emisja przy 370 nm; UVA2 - 20 W, zakres długości fali od 350 do 575 nm, największa emisja przy 350 nm. W kulturach prowadzonych za pomocą promieniowania UVA otrzymano najwyższe stężenie β-karotenu i karotenoidów, odpowiednio 63,20 μg/gs.m. i 0,97 mg/gs.m. W hodowlach napromieniowanych lampą VIS uzyskano niższe stężenie β-karotenu (43,60 μg/gs.m) i karotenoidów (0,83 mg/gs.m). Promieniowanie ultrafioletowe ze szczytem emisji promieniowania przy 370 nm promowało produkcję β-karotenu i karotenoidów w komórkach drożdży Rhodotorula mucilaginosa.(abstrakt oryginalny)

Słowa kluczowe

EN

Biotechnology; Microbiology

PL

Biotechnologia; Mikrobiologia

• Czasopismo: Nauki Inżynierskie i Technologie / Uniwersytet Ekonomiczny we Wrocławiu
• Rocznik: 2016
• Numer: nr 3 (22)
• Strony: 9--20

Twórcy

autor

Ludmiła Bogacz-Radomska

• Uniwersytet Ekonomiczny we Wrocławiu

autor

Joanna Harasym

• Uniwersytet Ekonomiczny we Wrocławiu

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Identyfikatory

DOI

10.15611/nit.2016.3.01